![]() ![]() González-Esparza D, Del Angel-Arroyo JA, Elvira-Hernández EA, Herrera-May AL, Aguilera-Cortés LA (2019) Design and modeling of a microfluidic device with potential application for isolation of circulating tumor cells. J Mol Diagn 15(2):149–157įachin F, Spuhler P, Martel-Foley JM et al (2017) Monolithic chip for high-throughput blood cell depletion to sort rare circulating tumor cells. Anal Chem 83(6):2301–2309ĭong Y, Skelley AM, Merdek KD, Sprott KM, Jiang C, Pierceall WE, Lin J, Stocum M, Carney WP, Smirnov DA (2013) Microfluidics and circulating tumor cells. ![]() Lab Chip 11:375–377ĭharmasiri U, Njoroge SK, Witek MA, Adebiyi MG, Kamande JW, Hupert ML, Barany F, Soper SA (2011) High-throughput selection, enumeration, electrokinetic manipulation, and molecular profiling of low-abundance circulating tumor cells using a microfluidic system. Cancer Treat Rev 36(Suppl 1):S1–S10ĭen Toonder J (2011) Circulating tumor cells: the grand challenge. Bioanalysis 2:1701–1710īouche O, Beretta GD, Alfonso PG, Geissler M (2010) The role of anti-epidermal growth factor receptor monoclonal antibody monotherapy in the treatment of metastatic colorectal cancer. Clin Cancer Res 10(20):6897–6904Īmasia M, Madou M (2010) Large-volume centrifugal microfluidic device for blood plasma separation. Biomicrofluidics 13:034112Īllard WJ et al (2004) Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases. The horizontal and vertical gaps between microposts have been optimized such that the DLD based device imposes minimal resistive imposition to the movement of cells, maintains unaltered pressure points distribution surrounding microposts and at outlets, amplifies throughput and provides superlative isolation efficiency and purity of extracted CTCs and WBCs.Īghilinejad A, Aghaamoo M, Chen X (2019) On the transport of particles/cells in high-throughput deterministic lateral displacement devices: Implications for circulating tumor cell separation. The obtained response after simulation study shows that the proposed device design separates out two distinct CTCs from WBCs through corresponding specified outlets at elevated sample inflow rate of 22 × 10 –6 kg/s and Re of 22.47 thus functioning appreciably at elevated value of throughput. Making use of a computation software COMSOL Multiphysics 5.4, simulated design of the proposed microfluidic device has been analyzed taking into account an infused blood sample comprising of massive CTCs branching from lung cancer site, CTCs branching from prostate cancer site and comparatively smaller WBCs of diametric sizes 22.5 µm, 10.64 µm and 12 µm correspondingly. In this study, a microfluidic device with a single sample inlet, two symmetrical buffer inlets and housing an asymmetric DLD array, has been presented that clearly distinguishes two distinctive CTCs from White blood cells (WBCs) using continuous flow through a single DLD array. Deterministic lateral displacement (DLD), being a tried and tested passive technique depending on uneven bifurcation of laminar flow traversing through a structured array of microposts, is a promising method in isolating cells of differing sizes. Circulating tumor cells (CTCs) are the cells, found in extremely few numbers in human blood branching out from the primitive tumor location, carried further by the circulation of blood passage, thus recognized as a major crucial bio-indicator for early and promising cancer cure and diagnosis. ![]()
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